SFC/EM Investigación

[t]Absence of XMRV and other MLV-related viruses in patients with Chronic Fatigue Syndrome[/t]
Clifford H. Shin1, Lucinda Bateman2, Robert Schlaberg1, Ashley M. Bunker3, Christopher J. Leonard1, Ronald W. Hughen4, Alan R. Light4, Kathleen C. Light4, and Ila R. Singh1,*

1 Department of Pathology, University of Utah, Salt lake City, Utah, 84112
2 Fatigue Consultation Clinic, Salt Lake City, Utah, 84102
3 ARUP Laboratories, Salt Lake City, Utah, 84108
4 Department of Anesthesiology, University of Utah, Salt Lake City, Utah, 84112

* Corresponding author: Mailing address: Emma Eccles Jones Medical Research Building, Department of Pathology, 15 North Medical Drive East, Suite #2100, Salt Lake City, UT 84112, Phone: (801) 213-3737, Fax: (801) 585-7376, Email: [email protected]

ABSTRACT
Chronic fatigue syndrome (CFS) is a multi-system disorder characterized by prolonged and severe fatigue that is not relieved by rest. Attempts to treat CFS have been largely ineffective primarily because the etiology of the disorder is unknown. Recently CFS has been associated with xenotropic murine leukemia virus-related virus (XMRV) as well as other murine leukemia virus (MLV)-related viruses, though not all studies have found these associations. We collected blood samples from 100 CFS patients and 200 self-reported healthy volunteers from the same geographical area. We analyzed these in a blinded manner using molecular, serological and viral replication assays. We also analyzed samples from patients in the original study that reported XMRV in CFS. We did not find XMRV or related MLVs, either as viral sequences or infectious virus, nor did we find antibodies to these viruses in any of the patient samples, including those from the original study. We show that at least some of the discrepancy with previous studies is due to the presence of trace amounts of mouse DNA in the Taq polymerase enzymes used in these previous studies. Our findings do not support an association between CFS and MLV-related viruses including XMRV and off-label use of antiretrovirals for the treatment of CFS does not seem justified at present.

enlace: http://jvi.asm.org/cgi/content/abstract/JVI.00693-11v1" onclick="window.open(this.href);return false;

Otro estudio negativo... este es delicado, porque los autores tienen mucho renombre... tendremos que esperar las diversas reacciones a ver...

Paciencia ... que es la madre de la ciencia... nunca mejor dicho

http://news.sciencemag.org/scienceinsid ... tml?ref=hp" onclick="window.open(this.href);return false;

The lead author of the contested Science paper, retrovirologist Judy Mikovits of the Whittemore Peterson Institute for Neuro-Immune Disease in Reno, Nevada, isn't persuaded by the Singh group's inability to detect XMRV in anyone. "I was astounded when I leaned that Ila [Singh] didn't find it," says Mikovits. "These are good scientists."

Mikovits says she remains confident that the 14 CFS patients she selected for Singh's group have XMRV in their bodies. "These people are infected," says Mikovits. "This study says nothing. We have complete confidence in every bit of the results in the Science paper. We don't think any of it is wrong. There is no evidence of contamination in our lab, and we have controlled for that all along."

Mikovits notes that Singh's group did not use the identical protocols for every analysis, and stresses that discrepancies between their labs may also reflect her own finding that XMRV levels vary in patients day to day. Singh counters that although some protocol differences exist, they worked closely with Mikovits' team to replicate the original work. Singh says the fact that they didn't find XMRV in any of these patients is significant. "She [Mikovits] pointed us toward patients that she had repeatedly tested positive," says Singh. "We should have found at least one that was positive. Not all of them would have gone negative on the day when a phlebotomist met with them."

He leído como respuesta al estudio, que se ha demostrado (en los estudios en Macacos Rhesus) que el XMRV desaparece de la sangre en poco tiempo, y que el mismo equipo de investigadores ha encontrado XMRV en los tejidos de enfermos de cáncer de próstata (aunque en sangre no salía - en los mismos enfermos). Hay quien se pregunta por qué no se hace el estudio directamente en tejido (como hace DeMeirleir).

Supongo que es como todo, hay que seguir esperando.

parece que de lo que se trata es de liarnos mas todavia... por qué no pueden simplemente limitarse a hacer el estudio con los métodos exactos que usaron en el WPI...? en este caso yo crei que lo habian hecho así, pero poco a poco van apareciendo mas datos, al final resultará que tampoco...

Aquí está la respuesta del WPI
viewtopic.php?p=5566#p5566" onclick="window.open(this.href);return false;