Fenotipo y funcionamiento de células NK, B y T en SFC
Publicado: 23 Mar 2013, 00:05
Seguimos con la búsqueda de biomarcadores inmunológicos para el Síndrome de Fatiga Crónica. En este estudio participan Alegre, Gª Quintana, Rigau y primeras figuras del IrsiCaixa. En el estudio, explican que, mientras que en números absolutos hay poca diferencia entre los grupos, existen características en el fenotipo y la proliferación de las células T y NK que clasifican claramente a los enfermos de SFC, que puede ser útil como biomarcador.
Lástima que se hagan estudios en la línea correcta, pero con sólo 22 enfermos. Estas conclusiones (o similares) aparecen también en estudios de 2010 y 2011 (no sé si anteriores). Si no se hacen estos mismos estudios con cohortes más amplias de enfermos, parece que caminemos en círculos, en lugar de avanzar...
[t]Screening NK-, B- and T-cell phenotype and function in patients suffering from Chronic Fatigue Syndrome[/t]
Marta Curriu, Jorge Carrillo, Marta Massanella, Josepa Rigau, José Alegre, Jordi Puig, Ana M Garcia-Quintana, Jesus Castro-Marrero, Eugènia Negredo, Bonaventura Clotet, Cecilia Cabrera and Julià Blanco
Journal of Translational Medicine 2013, 11:68 doi:10.1186/1479-5876-11-68
Published: 20 March 2013
Abstract (provisional)
Background
Chronic Fatigue Syndrome (CFS) is a debilitating neuro-immune disorder of unknown etiology diagnosed by an array of clinical manifestations. Although several immunological abnormalities have been described in CFS, their heterogeneity has limited diagnostic applicability.
Methods
Immunological features of CFS were screened in 22 CFS diagnosed individuals fulfilling Fukuda criteria and 30 control healthy individuals. Peripheral blood T, B and NK cell function and phenotype were analyzed by flow cytometry in both groups.
Results
CFS diagnosed individuals showed similar absolute numbers of T, B and NK cells, with minor differences in the percentage of CD4+ and CD8+ T cells. B cells showed similar subset frequencies and proliferative responses between groups. Conversely, significant differences were observed in T cell subsets. CFS individuals showed increased levels of T regulatory cells (CD25+/FOXP3+) CD4 T cells, and lower proliferative responses in vitro and in vivo. Moreover, CD8 T cells from the CFS group showed significantly lower activation and frequency of effector memory cells. No clear signs of T-cell immunosenescence were observed. NK cells from CFS individuals displayed higher expression of NKp46 and CD69 but lower expression of CD25 in all NK subsets defined. Overall, T cell and NK cell features clearly clustered CFS individuals.
Conclusions
Our findings suggest that alterations in T-cell phenotype and proliferative response along with the specific signature of NK cell phenotype may be useful to identify CFS individuals. The striking down modulation of T cell mediated immunity may help to understand intercurrent viral infections in CFS.
Enlace al pdf provisional (estudio completo): http://www.translational-medicine.com/c ... -11-68.pdf" onclick="window.open(this.href);return false;
Lástima que se hagan estudios en la línea correcta, pero con sólo 22 enfermos. Estas conclusiones (o similares) aparecen también en estudios de 2010 y 2011 (no sé si anteriores). Si no se hacen estos mismos estudios con cohortes más amplias de enfermos, parece que caminemos en círculos, en lugar de avanzar...
[t]Screening NK-, B- and T-cell phenotype and function in patients suffering from Chronic Fatigue Syndrome[/t]
Marta Curriu, Jorge Carrillo, Marta Massanella, Josepa Rigau, José Alegre, Jordi Puig, Ana M Garcia-Quintana, Jesus Castro-Marrero, Eugènia Negredo, Bonaventura Clotet, Cecilia Cabrera and Julià Blanco
Journal of Translational Medicine 2013, 11:68 doi:10.1186/1479-5876-11-68
Published: 20 March 2013
Abstract (provisional)
Background
Chronic Fatigue Syndrome (CFS) is a debilitating neuro-immune disorder of unknown etiology diagnosed by an array of clinical manifestations. Although several immunological abnormalities have been described in CFS, their heterogeneity has limited diagnostic applicability.
Methods
Immunological features of CFS were screened in 22 CFS diagnosed individuals fulfilling Fukuda criteria and 30 control healthy individuals. Peripheral blood T, B and NK cell function and phenotype were analyzed by flow cytometry in both groups.
Results
CFS diagnosed individuals showed similar absolute numbers of T, B and NK cells, with minor differences in the percentage of CD4+ and CD8+ T cells. B cells showed similar subset frequencies and proliferative responses between groups. Conversely, significant differences were observed in T cell subsets. CFS individuals showed increased levels of T regulatory cells (CD25+/FOXP3+) CD4 T cells, and lower proliferative responses in vitro and in vivo. Moreover, CD8 T cells from the CFS group showed significantly lower activation and frequency of effector memory cells. No clear signs of T-cell immunosenescence were observed. NK cells from CFS individuals displayed higher expression of NKp46 and CD69 but lower expression of CD25 in all NK subsets defined. Overall, T cell and NK cell features clearly clustered CFS individuals.
Conclusions
Our findings suggest that alterations in T-cell phenotype and proliferative response along with the specific signature of NK cell phenotype may be useful to identify CFS individuals. The striking down modulation of T cell mediated immunity may help to understand intercurrent viral infections in CFS.
Enlace al pdf provisional (estudio completo): http://www.translational-medicine.com/c ... -11-68.pdf" onclick="window.open(this.href);return false;
